Journal: Molecular Pharmaceutics
Article Title: Oxygen/Nitric Oxide Dual-Releasing Nanozyme for Augmenting TMZ-Mediated Apoptosis and Necrosis
doi: 10.1021/acs.molpharmaceut.4c00817
Figure Lengend Snippet: Apoptosis/necrosis assay and in vitro antitumor efficacy assay. (A) Apoptosis/necrosis ratios of cells under different conditions were analyzed by flow cytometry. Apoptotic cells were stained by Apopxin Green and necrotic cells were stained by 7-AAD. (B) In vitro antitumor efficacy assay. Cell viability of free TMZ, CCM-CeO 2 , and CCM-CeO 2 -TMZ was determined by the fluorescence intensity of Calcein AM.
Article Snippet: The dissociated cells were centrifuged and washed with DPBS and stained by a Cell Meter Apoptotic and Necrotic Multiplexing Detection Kit (AAT Bioquest) at room temperature for 30 min. After DPBS washing, a flow cytometer was used to analyze cells (Luminex Amnis Image Stream).
Techniques: In Vitro, Flow Cytometry, Staining, Fluorescence